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FLOW CYTOMETRY

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Flow cytometry provides rapid multi-parametric analysis of single cells in solution.

We use the multiple lasers and detectors on our high-end full spectrum flow cytometers to allow us to label cells with more than 30 individual fluorophores conjugated to antibodies, for immunophenotyping and characterisation. The ability to simultaneously measure multiple parameters on a cell-by-cell basis is one of the most powerful aspects of analytical flow cytometry.  

 

Aside from immunophenotyping there are many other applications of flow cytometry, including intracellular antigen expression (cytokine/chemokine production), cell cycle analysis, measurement of DNA and RNA content, apoptosis, viability, rare event analysis (stem cells, side population, dendritic cells), cell counting (absolute counts), fluorescent protein expression, mitochondrial membrane potential, cellular kinetics (proliferation), FRET, bead assays (CBAs), bacteria analysis and cell sorting. From immune cells, nanoparticles, microalgae and bacteria to parasite eggs, if the sample can be reduced to individual particles ranging from 0.1µm-60µm we can analyse and sort them at speeds of >10,000 cells per second.

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Our team ensure that the flow cytometers housed in the Hugh Green Technology Centre undergo daily quality control checks and are kept in optimal working order. We offer instrument support and troubleshooting tips, panel design, validation and implementation support, assistance with experiment optimisation such as sample preparation, troubleshooting and experimental design; data analysis, interpretation, troubleshooting and reporting.

FULL SPECTRUM FLOW CYTOMETRY 
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5-laser Cytek Auroras
  • 5-laser full spectrum analyser

  • Lasers: 351 nm (16 detectors), 405 nm (16 detectors), 488 nm (14 detectors), 561 nm (10 detectors), 638 nm (8 detectors)

  • Speed: sample acquisition of 10,000 events/sec

  • Capabilities: Spectral unmixing of highly-overlapping fluorophores, autofluorescence subtraction. Three instruments include plate sample loaders with mixing and temperature control

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IMMUNOASSAYS (LUMINEX)
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The Luminex is an instrument that enables multiplexing of different analytes in a single microplate well for biological assays similar to ELISA. 

 

Analytes of interest are sandwiched between coded beads and a reporter antibody, allowing quantification of analyte concentration. The Luminex 200 system housed at MIMR can analyse up to 80 analytes simultaneously, with minimal volume required (10-20uL) and a plate read time of approximately 45 minutes. The HGTC offers the service of running Luminex 200 assays.

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CONTACT US

Malaghan Institute of Medical Research
Gate 7, Victoria University
Kelburn Parade, Wellington

 

PO Box 7060
Newtown, Wellington 6242
New Zealand

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