CELL SORTING

BD INFLUX

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With a wide range of nozzle sizes, the BD Influx can analyse and sort a large range of particle sizes, with the sort set-up tailored to each sample type. A range of collection options are available, with capability of index and up to six-way sorting.  Fully sterilised sorts are also an option. Our team can manage the running of the sorter, and will handle all aspects of the sort aside from sample preparation – the user simply brings their prepared sample, assists in gating set-up, then collects their sorted product upon completion of the sort.

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We have experience sorting bacteria, yeast, DCs, T cells, macrophages, mast cells, eosinophils, neutrophils, primary tumour samples, side-populations, gut and skin samples, parasitic nematode eggs, vaginal cells, buccal cells, to name a few.

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BD Influx (6-laser)

• Stream in air sorter

• Lasers: 355nm, 405nm, 445nm, 488nm, 552nm and 640nm

• 21 colour + 3 scatter, including the Small Particle Detector (2 FSC PMTs)

• Capabilities: 

  • 24 parameters (up to 22 colours, 2-3 scatter, including polarised FSC)

  • Up to 30,000 events/sec

  • Supports 2-, 4- and 6-way cell sorting 

  • Fully enclosed in a certified Class II hood and sort sterility is a high priority. Cooled sample delivery and collection

  • 1.7mL, 5mL, 15mL and 50mL tube holders for sort samples, plates (6-384well), slides

  • Index sorting

  • Computerised Cell Deposition Unit (CCDU) allowing for single-cell sorting into 384 well-, 96 well- and PCR-plates, or onto microscope slides

  • 5 Nozzle Sizes: 70µm, 86µm, 100µm, 140µm and 200µm 

  • Highly-configurable

  • We have experience sorting bacteria, yeast, DCs, T cells, macrophages, mast cells, eosinophils, neutrophils, primary tumour samples, gut and skin samples, parasitic nematode eggs, vaginal cells, buccal cells, to name a few

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Full Spectrum Cell Sorter: Cytek Aurora CS

• Cuvette spectral sorter

• Lasers: 351 nm (16 detectors), 405 nm (16 detectors), 488 nm (14 detectors), 561 nm (10 detectors), 638 nm (8 detectors). Three scattering channels and 64 fluorescence channels.

• Capabilities: 

  • Sort up to 6 ways into 1.5 mL tubes, or up to 4 ways into 5 mL tubes. 

  • 40 colours demonstrated including fluorochromes with emission spectra in close proximity to each other.  

  • Customizable nozzle settings and sort modes

  • Automated drop delay, sort monitoring, and clog detection for a reliable sorting experience.

  • Extract autofluorescence and improve resolution of highly autofluorescent samples.

  • Choose from a variety of sample input and collection devices including 5 and 15 mL tubes for input and 384 and 96-well plates, 1.5, 5, and 15 mL tubes for collection.

  • Index sorting