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CELL SORTING

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Our state-of-the-art fluorescence activated cell sorting equipment can sort anything from bacteria to nematode eggs.

BD INFLUX

With a wide range of nozzle sizes, the BD Influx can analyse and sort a large range of particle sizes, with the sort set-up tailored to each sample type. A range of collection options are available, with capability of index and up to six-way sorting.  Fully sterilised sorts are also an option. Our team can manage the running of the sorter, and will handle all aspects of the sort aside from sample preparation – the user simply brings their prepared sample, assists in gating set-up, then collects their sorted product upon completion of the sort.

We have experience sorting bacteria, yeast, DCs, T cells, macrophages, mast cells, eosinophils, neutrophils, primary tumour samples, side-populations, gut and skin samples, parasitic nematode eggs, vaginal cells, buccal cells, to name a few.

BD Influx (6-laser)
  • Stream in air sorter

  • Lasers: 355nm, 405nm, 445nm, 488nm, 552nm and 640nm

  • 21 colour + 3 scatter, including the Small Particle Detector (2 FSC PMTs)

  • Capabilities: 

    • 24 parameters (up to 22 colours, 2-3 scatter, including polarised FSC)

    • Up to 30,000 events/sec

    • Supports 2-, 4- and 6-way cell sorting 

    • Fully enclosed in a certified Class II hood and sort sterility is a high priority. Cooled sample delivery and collection

    • 1.7mL, 5mL, 15mL and 50mL tube holders for sort samples, plates (6-384well), slides

    • Index sorting

    • Computerised Cell Deposition Unit (CCDU) allowing for single-cell sorting into 384 well-, 96 well- and PCR-plates, or onto microscope slides

    • 5 Nozzle Sizes: 70µm, 86µm, 100µm, 140µm and 200µm 

    • Highly-configurable

    • We have experience sorting bacteria, yeast, DCs, T cells, macrophages, mast cells, eosinophils, neutrophils, primary tumour samples, gut and skin samples, parasitic nematode eggs, vaginal cells, buccal cells, to name a few

Full Spectrum Cell Sorter: Cytek Aurora CS
  • Cuvette spectral sorter

  • Lasers: 351 nm (16 detectors), 405 nm (16 detectors), 488 nm (14 detectors), 561 nm (10 detectors), 638 nm (8 detectors). Three scattering channels and 64 fluorescence channels.

  • Capabilities: 

    • Sort up to 6 ways into 1.5 mL tubes, or up to 4 ways into 5 mL tubes. 

    • 40 colours demonstrated including fluorochromes with emission spectra in close proximity to each other.  

    • Customizable nozzle settings and sort modes

    • Automated drop delay, sort monitoring, and clog detection for a reliable sorting experience.

    • Extract autofluorescence and improve resolution of highly autofluorescent samples.

    • Choose from a variety of sample input and collection devices including 5 and 15 mL tubes for input and 384 and 96-well plates, 1.5, 5, and 15 mL tubes for collection.

    • Index sorting

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CONTACT US

Malaghan Institute of Medical Research
Gate 7, Victoria University
Kelburn Parade, Wellington

 

PO Box 7060
Newtown, Wellington 6242
New Zealand

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