TRAINING AND SERVICES
We offer expert guidance on designing experiments tailored to specific analyses, as well as interpreting data post-experiment.
Our consultancy services include:
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Advising on the choice between single-cell and bulk RNA sequencing
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Determining the optimal number of samples for robust downstream analysis
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Supporting differential expression analysis from cDNA sequencing data
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Exploring various approaches to investigate genetic variation
SINGLE-CELL RNA SEQUENCING
We offer training and skilled-user access to the 10x Chromium iX platform. We also provide comprehensive guidance in experimental design and will prepare single-cell RNA libraries on your behalf on both the 10x Chromium iX and BD Rhapsody platforms.
These libraries can be sequenced in-house or be sent to the sequencing facility of your choice. Our Data Science team is available to support you in the analysis of sequencing results, including running workloads on our on-premises high-performance computing.
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COMPUTATION AND APPLICATION
Our team can assist with:
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Creating and supporting scripts to automate repetitive tasks
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Designing applications for exploratory data analysis
LARGE-SCALE DATA ANALYSIS
We work together with you and our Data Science core to process and analyze genomic data. This integration enables us to incorporate the bioinformatic outcomes into our wet-lab processes, which aids in optimizing protocols and developing follow on experiments for your project.
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RNA PLATFORM
During the pandemic, RNA technology has come to the forefront for its safety, efficacy and rapid development potential.
We have established and are scaling up the three major components required for the development and production of RNA therapeutics:
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​Plasmid DNA construct design and purification
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mRNA synthesis and purification
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Lipid nanoparticle encapsulation
If you are interested in accessing RNA vaccines and therapeutics for your research, please get in touch with: rmckenzie@malaghan.org.nz
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INSTRUMENTATION
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The Hugh Green Technology Centre has access to two Lipid Nanoparticle Assemblers, the Ignite and the Blaze from Precision Nanosystems Inc. These are essential instruments for the generation of RNA therapeutics as they allow for generation of liquid nanoparticles of a consistent size and low polydispersity. The same process used in pre-clinical scale production of mRNA/LNPs can be scaled up to larger manufacturing production, saving time bringing a product to the market.
The Hugh Green Technology Centre and the National RNA Platform are working together to implement the technologies housed in the genomics core to support the successful development of RNA vaccines, therapeutics and their translation to the clinic.
For more information visit the Ribonucleic Acid (RNA) Development Platform website:
https://www.wgtn.ac.nz/ferrier/research/rnaplatform
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