SEQUENCING LIBRARY PREPARATION
We offer comprehensive library preparation services ranging from bulk RNA-seq experiments to single-cell-multiomics. We also have a spatial transcriptomics platform that couples single-cell transcriptome data with bioimaging analysis.
Our advanced cell sorting capabilities enable the selection of specific cell subsets of interest, significantly reducing sequencing costs. Our facility includes a dedicated space for in-house genomic workflows and library generation. Libraries are prepared and quality controlled in-house before being sent to an external sequencing provider of your choice.
SINGLE CELL GENOMICS
10x Genomics Chromium X
We have enhanced our single-cell RNA sequencing capabilities with the acquisition of the 10x Genomics Chromium X instrument. The 10x genomics workflow for scRNAseq enables 3’ and 5’ WTA sequencing, BCR/TCR sequencing, scATACseq, and CRISPR screening. This system can capture both cells and nuclei, it is compatible with fresh and frozen samples and with FFPE-fixed cells if using the probe-based Flex system, which can capture about 19,000 genes and be customised with additional probes. The Chromium X allows on-chip multiplexing of up to 4 samples together. Simultaneous antibody-oligo capture assays can be incorporated to simultaneously interrogate the transcriptome and proteome.
BD Rhapsody
The BD Rhapsody Express is used for capturing single cells for single-cell RNA sequencing via a gentle microwell capture procedure. Libraries are prepared and quality controlled in-house before being sent to an external sequencing provider of your choice. Our Data Science team can support data analysis, or clients can perform it independently.
BD Rhapsody Express can be paired with optional data QC by Seven Bridges. BD recommends SeqGeq, a desktop bioinformatics platform with an intuitive interface, for complex scRNA-seq analysis.
SPATIAL TRANSCRIPTOMICS
Visium HD with CytAssit
Spatial discovery with Visium HD Spatial Gene Expression.
See your tissue in high definition:
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whole transcriptome analysis at single cell-scale resolution.
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continuous tissue coverage.
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best-in-class data powered by innovative probe-based chemistry and a Visium CytAssist-enabled workflow.
The Visium HD features 2µm barcoded capture squares, while the standard Visium offers a 100µm spot-to-spot distance. Both allow for whole transcriptome analysis (WTA) overlaid on H&E or immunofluorescent staining. Capture areas of 6.5 x 6.5 mm and 11 x 11 mm are available.
The Visium HD, detects approximately 18,000 unique genes and is available for mouse and human samples. The classic Visium offers polyA tail capture and probe-based assays, with some assays compatible with antibody-oligo capture. Depending on the assay, fresh frozen, fixed frozen, or FFPE samples can be used. The CytAssist Instrument optimises tissue transfer from standard glass slides to the Visium capture slides and allows for better resolution.