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DROPLET DIGITAL PCR

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The Hugh Green Technology Centre offers advanced droplet digital PCR (ddPCR) capabilities utilising the BIORAD QX200 Droplet Digital PCR System.

This method leverages water-oil emulsion droplet technology, wherein a sample is partitioned into 20,000 droplets, with PCR amplification occurring in each individual droplet. The ddPCR technology employs reagents and workflows analogous to those used in standard TaqMan probe-based assays, with massive sample partitioning being a critical feature of the ddPCR technique.

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ddPCR is particularly advantageous in scenarios where PCR inhibitors may be present, such as in faecal samples. It has been effectively used in wastewater testing for COVID-19 detection, where both template quality and quantity are low, and PCR inhibitors are prevalent. A significant benefit of ddPCR over qPCR is the elimination of the need for a standard curve, as ddPCR directly counts template molecules, providing an absolute measure of concentration.

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The ddPCR System can be used to:

  • detect rare DNA target copies

  • determine copy number variation

  • measure gene expression levels.

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Applications of the QX200 Droplet Digital PCR System include:

  • cancer biomarker studies and copy number variation 

  • pathogen detection

  • next generation sequencing

  • gene expression analysis

  • environmental monitoring

  • food testing.

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CONTACT US

Malaghan Institute of Medical Research
Gate 7, Victoria University
Kelburn Parade, Wellington

 

PO Box 7060
Newtown, Wellington 6242
New Zealand

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