SEQUENCING
The Hugh Green Technology Centre utilises the MinION and PromethION P2 Solo from Oxford Nanopore Technologies for long-read sequencing.
OXFORD NANOPORE LONG-READ SEQUENCING
We use the MinION and PromethION P2 Solo from Oxford Nanopore Technologies for long-read sequencing.
Nanopore sequencing involves passing a long polymer through a protein pore using an electric current to detect changes in translocation speed, which vary based on the DNA base. This rapid, continuous method allows for efficient DNA sequencing.
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The MinION and PromethION P2 Solo sequencers use consumable flow cells that contain a polymer membrane embedded protein pores (nanopores). An electrical potential across the membrane drives ion flow through the nanopore, each type of DNA base creates a different signal through the pore.​
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Choosing your flow cell depends on the desired depth of sequencing and application. The MinION flow cell contains 512 nanopores and yields up to 48 gigabases from a 72 hour run. ​The PromethION flow cell contains 2,675 nanopore channels and yields up to 290 gigabases from a 72 hour run.
A symbolic representation of a chimeric read captured by an ONT MinION DNA sequencer (A), together with the annotated raw signal (B), containing three separate hairpin events within the same base-called sequence. Hairpin features can be seen by eye within the signal; attached barcodes demonstrate that this sequence was formed from an overnight ligation reaction. White R., et al. F1000 Research 2017.