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HISTOLOGY

Amy Shepherd Image-1.tiff
We offer a workflow from sample processing, staining (histology) and image acquisition (microscopy) through to data analysis, providing publication-ready results.

We have extensive experience in histological techniques including formalin-fixed, paraffin embedding (FFPE) of tissue samples and cryosectioning techniques. We can provide generic histological staining such as H&E, PAS, sirius red and masson trichome and we have experience with fluorescence in-situ hybridization (FISH). We routinely perform multicolour direct-conjugated immunofluorescent staining on sectioned samples.

TISSUE PROCESSOR

 

Tissue processing is required to adequately dehydrate, clear and infiltrate tissue with paraffin wax during the production of FFPE tissue. We use the Citadel 2000 which is programmed to move samples dehydrate tissue in ascending concentrations of ethanol followed by the slow clearing of tissue with xylene, preparing it for hydrophobic wax infiltration.

EMBEDDING TABLE

 

The Epredia HistoStar Embedding Workstation is a key piece of equipment used in the generation of formalin-fixed paraffin-embedded tissue blocks. Once tissue has undergone FFPE processing, samples are submerged in molten paraffin wax upon the hot plate, and allowed to solidify on the cold table. These samples can be de-molded and sectioned using a microtome.

MICROTOME

 

The Micron Rotary Microtome HM325 allows for the fixed and incremental generation of very thin sections of FFPE tissue samples. These thin tissue sections can then be stained with immunohistochemical markers and histological stains, and imaged under microscopes.

 

CRYOSTAT

 

The UM-C52040 Cryostat consists of a microtome within a refrigerated cabinet and is designed to operate between 5°C to -40°C. A cryostat is used to produce very thin sections of tissue that have been embedded in a cryoprotective medium and snap-frozen using the Gentle Jane system. Cryosections can be stained with immunohistochemical and immunofluorescent markers and imaged under microscopes.

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CONTACT US

Malaghan Institute of Medical Research
Gate 7, Victoria University
Kelburn Parade, Wellington

 

PO Box 7060
Newtown, Wellington 6242
New Zealand

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