GROW YOUR FLOW PROGRAMME

The first step to mastering cytometry. Join us for an engaging introduction to flow cytometry, where you’ll learn the essentials; from instrument hardware and fluorochromes to data visualisation, gating strategies, and experimental controls. Build a strong foundation and start growing your flow expertise today.

Mastering sample preparation and staining basics. Take your next step in flow cytometry with this expansion module focused on preparing samples for success. Learn essential techniques for cell handling, basic staining protocols, and tips to ensure high-quality data. Build confidence in the fundamentals that set the stage for accurate and reproducible cytometry and cell sorting experiments.

Hands-On data analysis with SpectroFlo. Dive into your first fully interactive data module. Learn how to analyse flow cytometry results using SpectroFlo software; begin by unmixing a multicolour assay before learning how to interpret plots and apply gating strategies. This practical session builds confidence in turning raw data into clear results. (software demo license provided if you do not already have a license).

Advanced data analysis with FlowJo. Expand your skills by revisiting key concepts from the previous module in a new context: FlowJo. This powerful third-party software offers sophisticated tools for deeper analysis beyond acquisition. Learn gating, data visualisation, reporting, batching, and strategies to unlock richer insights from your experiments. (software demo license provided if you do not already have a license).

Designing experiments and planning controls. Take your cytometry skills to the next level by learning how to plan experiments with intention. This module covers the critical details of experimental design and addresses the differences between instrument and assay controls. Don’t know what an FMO is for? Find out this and so much more to ensure your results are robust and reproducible.

Troubleshooting real-world cytometry data. Put your skills to the test with a dataset full of everyday challenges. This module combines a lecture followed by a hands-on workshop. With instructional guidebook, the workshop will help you identify and resolve common issues in cytometry assays. Learn practical strategies for cleaning, correcting, and interpreting data so you can confidently tackle problems in your own experiments.

Pushing the limits with small particle analysis. Ready to explore the world of small particles, right there on the edge of cytometer sensitivity? This module dives into the challenges of small particle analysis; where precision and rigour are essential. Learn best practices for instrument care and setup, experimental controls, sample preparation, and instrument characterisation for quantitative measurements. Discover what it takes to verify results at the limits of detection and how to set yourself up for success in this powerful niche of cytometry.

Science or art? Panel design and optimisation. Designing the perfect panel is both an art and a science. In this module, we cover the fundamentals of panel design, including fluorochrome selection, brightness, uniqueness, and spread. Then, you will work collaboratively in a group-based design activity before diving into the critical next step: panel optimisation. Panel optimisation puts the science back into the art of panel design. Here you will learn tips and tricks for managing unexpected output when your multicolour sample does not recapitulate the biology you expect.

The why's, when's and how's of titration. Understand why titration matters and how it impacts your data quality. This module covers the principles of titration; why, when, and how to do it, followed by a hands-on analysis session in FlowJo. Learn to interpret a titration series and select an appropriate titre for your assay. (software demo license provided if you do not already have a license).

Unlocking high-dimensional analysis. Step into the world of advanced cytometry analytics. This module begins with a lecture on why high-dimensional analysis matters, the tools for dimensionality reduction and clustering, and how to turn complex results into meaningful visualisations. Then, apply what you’ve learned in a hands-on workshop using a guidebook and dataset designed to help you master these powerful techniques in OMIQ. (software demo license provided if you do not already have a license).

Mastering the unmixing wizard. Take your troubleshooting skills to the next level with this advanced module focused on spectral unmixing (principles apply to compensation as well). Learn how to set up controls correctly, gate for accurate fluorochrome definition, and apply practical hacks and shortcuts to make unmixing as smooth and efficient as possible. Elevate your confidence and precision in spectral analysis.

Reporting results and creating publication-ready figures. Learn how to elevate your flow cytometry reporting and figure preparation. This module explores best practices rooted in MiFlowCyt standards and offers practical advice for creating clear, impactful visualisations. Gain the skills to present your data with accuracy and professionalism, ensuring your figures match the quality of your experimental data.

Tackling autofluorescence in mammalian samples. Autofluorescence is no longer just a background nuisance; in the era of spectral cytometry, it’s a measurable component that can unlock deeper insights. This module explores common analytical challenges in mammalian samples, teaching you how to characterise, manage, and even leverage autofluorescence for better data. Learn best practices for panel design, controls, setup, and interpretation to turn complexity into clarity.

Optimising assays for rigour and reproducibility. In this final module, gain deep insight into designing experiments that stand the test of time. Learn best practices for longitudinal studies and strategies to manage variability across multiple runs. Discover how to optimise your assays for consistency, accuracy, and reproducibility; ensuring your data remains robust and reliable.

Grow Your Flow Graduation and celebration.